NATURE OF PROTEINS INTRITICALEAND ITS PARENTAL SPECIES: II. GEL FILTRATION AND DISC ELECTROPHORESIS RESULTS

Abstract

The compositions and identity of protein components in the water, salt solution, 70% ethanol, and acetic acid solution extracts of the endosperm from one line of Triticale, its parents, and one cultivar of hard red spring wheat were investigated by gel filtration and disc electrophoresis. Albumins, globulins, and gliadins, as defined by their solubility, molecular weight, and electrophoretic mobility, were obtained by gel filtration on Bio-Gel P-150. Approximate molecular weights determined from elution volumes were 1 × 104to 3 × 1045 × 104to 9 × 104, and above 1.5 × 105for albumins and globulins, gliadins and glutenins, respectively. Each gel-filtration fraction contained several protein components as determined by disc electrophoresis. Quantitative distribution of fractions varied with species. For each fraction the amount for Triticale was intermediate between the amounts for the parental species. The gel-filtration spectrum for Triticale was similar to that for the hard red spring wheat. However, disc electrophoretic patterns for fractions obtained by gel filtration for the two species were quite different.