Author:
Songsasen N.,Martino A.,Leibo S. P.,Walmsley S.,Pollard J. W.,Buckrell B. C.
Abstract
A total of 104 oocytes were aspirated from 11 mature ewes and 4 prepubertal lambs. After in vitro maturation, fertilization and culture, 11 of the 104 oocytes developed into blastocysts. These were cryopreserved in 1.5 M ethylene glycol. After being thawed, all were transferred into four recipients, two of which became pregnant and delivered a normal lamb. Key words: Oocyte, cryopreservation, in vitro maturation and fertilization, sheep, embryo
Publisher
Canadian Science Publishing
Subject
Animal Science and Zoology,Food Animals
Cited by
5 articles.
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