Response of three Brassica species to high temperature stress during reproductive growth

Abstract

The effect of short periods of high temperature stress on the reproductive development and yield of three Brassica species were studied in a growth chamber experiment conducted for 2 yr. Two genotypes from Brassica juncea L. and one each from B. napus L. and B. rapa L. were grown under day/night temperatures of 20/15 °C till early flowering or early pod development, subjected to high temperature stress of 28/15 °C or 35/15 °C for 7 d and then allowed to recover at 20/15 °C. Species differed in optimum temperatures, with B. juncea and B. rapa having higher optimum temperature than B. napus. Dry matter was unaffected by moderate temperature stress, while it was reduced by high temperature stress. The 35/15 °C treatment was injurious to reproductive organs at different developmental stages of all three species. High temperatures at flowering affected yield formation more than high temperature at pod development. On the main stem, mean seed yield reduction due to heat stress was 89%, but partial compensation by pods on the branches reduced mean per-plant seed yield decrease to 52%. Reduction in fertile pods (not total pod number), thousand seed weight and seeds per pod were responsible for the reduced seed yield. Brassica rapa was more sensitive to heat stress than B. napus and B. juncea. Although observation did not indicate the exact developmental phase when the reproductive organs were susceptible to heat stress, pods that passed a critical threshold developmental phase tolerated heat stress, which explained the smaller effect of high temperature stress at pod development. A direct temperature effect on reproductive organs appeared to be responsible for the reduction in yield. All genotypes began to recover from the stress by continuing flowering after returning to 20/15 °C. Brassica napus was least able to recover from severe stress at flowering, as evidenced by the formation of many abnormal pods during recovery. Per-plant yield response of canola-quality B. juncea line J90-4316 was similar to oriental mustard Cutlass. Thus, heat stress effect depends on the growth stage of canola and mustard and Brassica species differ in heat stress response. Key words: Brassica species, napus, rapa, juncea, heat stress, yield, pod number