PROTEIN FRACTIONS AND LYSINE DISTRIBUTION IN BARLEY LINES DERIVED FROM HIPROLY

Abstract

Two high lysine barley segregants, one with a shrunken and one with a plump endosperm, were selected from an F5 population derived from the second backcross of adapted germ plasm to the non-recurrent donor parent, Hiproly. Seed protein of the two segregants and parental checks was classified by solubility in potassium sulfate, ethanol, and sodium hydroxide. Lysine content of the meal, residual meal, and protein fractions was determined with an amino acid analyzer. The data showed that lysine content of the meal protein was 13% higher for the plump and 12% higher for the shrunken segregant compared to the normal parent. The proportion of the potassium sulfate-soluble protein fraction and the lysine content of this fraction were both higher in the high lysine genotypes than in the normal lysine parent. On the average, the three solvents extracted 82.4% of the meal nitrogen and with the residual nitrogen, recovery was 96.8%. Lysine recovery from the three protein fractions and the isoluble residue averaged 76.9% of the lysine content of the meal.