Effects of low-temperature stress on development of winter crown rot in first-year alfalfa

Abstract

The effects of sub-lethal low temperatures on predisposing first-year alfalfa plants to infection by the low temperature basidiomycete (LTB), and the effects of sub-lethal winter crown rot levels on alfalfa cold hardiness were evaluated in controlled environment and field studies. Alfalfa, subjected to the sublethal stress temperature of –7.5 °C for 1 to 5 wk and inoculated with LTB, exhibited higher mortality, lower shoot dry weights, and higher winter crown rot levels than alfalfa similarly inoculated but non-stressed. Inoculation and incubation of alfalfa with the LTB fungus for 4 wk decreased the freezing resistance of cv. Barrier and cv. Peace alfalfa by 26 and 32%, respectively, compared with the non-inoculated controls. Following inoculation and incubation with LTB, stress temperatures between −6 and −15 °C increased LTB induced mortality in Barrier by 17–54% and in Peace by 10–75%. These results demonstrate that sub-lethal low-temperature stress can act synergistically with winter crown rot pathogen to reduce survival and yield in first-year alfalfa. Key words: Medicago sativa L., alfalfa, low temperature, winter crown rot, Coprinus psychromorbidus