Molecular Genetic Diagnostics and Features of the Species Structure of the Pathogenic Flora in Chlamydia and Mycoplasma Infection of the Respiratory Tract in Children

Abstract

Objective : to determine the features of the infection caused by Сhlamydia trachomatis, Chlamydia pneumoniae, Mycoplasma hominis, Mycoplasma pneumoniae in children with bronchitis and pneumonia and to improve the methodology of molecular genetic diagnostics of the respiratory infections caused by chlamydia and mycoplasma.Material and methods. 272 children aged 0-17 diagnosed with bronchitis, pneumonia, were examined for the presence of chlamydia-mycoplasma respiratory tract infection by Realtime PCR.Results. The study has revealed a high proportion of chlamydia and mycoplasma associations (23 %) with the predominance of Mycoplasma pneumoniae - 73 %. The identification of the associations Сhlamydia trachomatis , Сhlamydia pneumoniae , Mycoplasma hominis , Mycoplasma pneumoniae in bronchitis and pneumonia in the children was optimized by the use of Realtime PCR with Chlamydiaceae- and Mycoplasma-specific primers.Conclusion . To confirm the etiological role of chlamydia and mycoplasma in the development of respiratory pathology in children, it is promising to apply the specific and highly sensitive method of molecular genetic diagnostics - Real-time PCR.