TESTING OF CD107A MARKER EXPRESSION FOR THE ASSESSMENT OF ANTITUMOR ACTIVITY OF CYTOTOXIC LYMPHOCYTES-Reference-Cited by-同舟云学术

TESTING OF CD107A MARKER EXPRESSION FOR THE ASSESSMENT OF ANTITUMOR ACTIVITY OF CYTOTOXIC LYMPHOCYTES

Published:2011-12-28 Issue:2S Volume: Page:22-24
ISSN:2708-6011
Container-title:Health and Ecology Issues
language:
Short-container-title:jour

Author:

Vashkevich E. P.¹,Shman T. V.¹

Affiliation:

1. Republican Research Center for Pediatric Oncology and Hematology

Abstract

The assessment of antitumor activity of cytotoxic lymphocytes is an important indicator of their functional status in cellular immunotherapy. Testing of CD107a marker expression by the method of flow cytofluorimetry has been widely lately used for these purposes. The research that we carried out to determine the antitumor activity of mononuclear lymphocytes in peripheral blood including those stimulated by interleukine-2 showed that incubation of mononuclear lymphocytes in peripheral blood in the presence of К-562 tumor line was conducive to the reliable increase in rates of natural killer and killer-like cells expressing CD107a. A straight correlation between CD107a+killer cells percentage and the number of the dead targets in the test with the use of CFSE fluorescent label was detected. The assessment method of CD107a expression makes it possible to measure the cytotoxic activity in a cell in a concrete population of effectory cells and may be used alongside with standard methods for target-cell lysis.

Publisher

Gomel State Medical University

Reference9 articles.

1. BIBLIOGRAFIChESKII SPISOK

2. Sensitive and viable identification of antigen-specific CD8+ T-cells by a flow cytometric assay for degranulation / M. R. Betts [et al.] // J. of Immunol. Methods. - 2003. - Vol. 281. - P. 65-78.

3. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labeled allogeneic target cells in vitro: inhibition by isoantibody and by drugs / K. T. Brunner [et al.] // Immunology. - 1968. - Vol. 14. - P. 181-196.

4. A novel flow cytometric assay for evaluating cell-mediated cytotoxicity / M. W. Burkett [et al.] // J. Immunother. - 2005. - Vol. 28, № 4. - P. 396-402.

5. Fisher, K. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity / K. Fisher, A. Mackensen // Methods. - 2003. - Vol. 31. - P. 135-142.