Affiliation:
1. Gomel State Medical University
Abstract
Objective: to study the theoretical basis and give the experimental validation of the efficiency of hepatocyte isolation by the method of enzymatic perfusion of the liver. Material and methods. Wistar rats at a mass of 200 g, isolation of hepatocytes by enzymatic perfusion of the liver, light microscopy, assessment of functional cell capacity to synthesize and accumulate glycogen. Results. The article shows the high efficiency of the method of the 2-step adapted enzymatic perfusion of the liver to isolate hepatocytes. The culture of viable hepatocytes with preserved morphological and functional properties was obtained.
Publisher
Gomel State Medical University
Reference14 articles.
1. Fitzpatrick, E. Human hepatocyte transplantation: state of the art / E. Fitzpatrick, R. R. Mitry, A. Dhawan // J. Intern. Med. - 2009. - Vol. 266. - P. 339-357.
2. Primenenie izolirovannykh kletok pecheni s tsel'yu lecheniya bol'nykh s pechenochnoi nedostatochnost'yu razlichnogo geneza / I. I. Shimanko [i dr.] // Problemy kriobiologii. - 1995. - № 2. - S. 49-52.
3. Shumakov, V. I. Lechenie tyazheloi pechenochnoi nedostatochnosti perfuziei krovi bol'nogo cherez vzves' kriokonservirovannykh gepatotsitov / V. I. Shumakov, V. S. Arzumanov, N. A. Onishchenko // Khirurgiya. - 1990. - № 2. - S. 113-116.
4. Fausto, N. The role of hepatocytes and oval cells in liver regeneration and repopulation / N. Fausto, J. S. Campbell // Mech. Dev. - 2003. - Vol. 120. - P. 117-130.
5. Cell-based therapy for liver diseases / C. Di Campli [et al.] // 7 Eur. Rev. Med. Pharmacol. Sci. - 2003. - Vol. 7. - P. 41-44.