Comparison of different DNA detection options for TTV, TTMDV, and TTMV viruses

Abstract

Objective. To compare different variants of TTV, TTMDV, and TTMV DNA detection in the blood plasma of patients with various liver diseases and in individuals without signs of liver disease.Materials and methods. To detect TTV TTMDV, and TTMV DNA, the PCR method was used.Results. A fairly high frequency of TTV virus DNA detection was found using different laboratory approaches. The frequency of TTV DNA detection was significantly highest when using the primers for the non-coding region UTR – 77.3 % compared with the coding region ORF1 – 38.4 % (p < 0.001) and the commercial kit 53 % (p < 0.005).Conclusion. TTV DNA is detected significantly more often in patients with liver diseases compared with healthy individuals (90.3 % and 65.6 % in the UTR region and using the commercial kit, respectively), TTMV DNA (83.9 % – UTR region) and mixed DNA of the TTV + TTMDV + TTMV viruses (62.4 % – UTR region). To standardize the DNA detection of the TTV family viruses by the PCR method, it is advisable to create a panel of sera containing reliably positive and negative samples.