Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos
Author:
Affiliation:
1. Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 770-8513, Japan
Publisher
Japanese Society of Animal Reproduction
Subject
Animal Science and Zoology
Link
https://www.jstage.jst.go.jp/article/jrd/65/3/65_2018-116/_pdf
Reference29 articles.
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2. 2. Galli C, Perota A, Brunetti D, Lagutina I, Lazzari G, Lucchini F. Genetic engineering including superseding microinjection: new ways to make GM pigs. Xenotransplantation 2010; 17: 397–410.
3. 3. Tan W, Proudfoot C, Lillico SG, Whitelaw CB. Gene targeting, genome editing: from Dolly to editors. Transgenic Res 2016; 25: 273–287.
4. 4. Laible G, Wei J, Wagner S. Improving livestock for agriculture - technological progress from random transgenesis to precision genome editing heralds a new era. Biotechnol J 2015; 10: 109–120.
5. 5. Keefer CL. Artificial cloning of domestic animals. Proc Natl Acad Sci USA 2015; 112: 8874–8878.
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