Off- and on-target effects of genome editing in mouse embryos
Author:
Affiliation:
1. Experimental Animal Division, RIKEN BioResource Research Center, Ibaraki 305-0074, Japan
2. Gene Engineering Division, RIKEN BioResource Research Center, Ibaraki 305-0074, Japan
Publisher
Japanese Society of Animal Reproduction
Subject
Animal Science and Zoology
Link
https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-128/_pdf
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3. 3. Willi M, Smith HE, Wang C, Liu C, Hennighausen L. Mutation frequency is not increased in CRISPR-Cas9-edited mice. Nat Methods 2018; 15: 756–758.
4. 4. Iyer V, Boroviak K, Thomas M, Doe B, Riva L, Ryder E, Adams DJ. No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice. PLoS Genet 2018; 14: e1007503.
5. 5. Anderson KR, Haeussler M, Watanabe C, Janakiraman V, Lund J, Modrusan Z, Stinson J, Bei Q, Buechler A, Yu C, Thamminana SR, Tam L, Sowick MA, Alcantar T, O’Neil N, Li J, Ta L, Lima L, Roose-Girma M, Rairdan X, Durinck S, Warming S. CRISPR off-target analysis in genetically engineered rats and mice. Nat Methods 2018; 15: 512–514.
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