Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
Author:
Affiliation:
1. IVF Namba Clinic, Osaka 550-0015, Japan
2. Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
3. IVF Osaka Clinic, Osaka 577-0012, Japan
4. HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan
Publisher
Japanese Society of Animal Reproduction
Subject
Animal Science and Zoology
Link
https://www.jstage.jst.go.jp/article/jrd/62/6/62_2016-112/_pdf
Reference45 articles.
1. 1. Suarez SS. Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction. Reprod Fertil Dev 2007; 19: 103–110.
2. 2. Morrell J, Rodríguez-Martínez H. Biomimetic techniques for improving sperm quality in animal breeding: a review. The Open Andrology J. 2009; 1: 1–9.
3. 3. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003; 1: 108–110.
4. 4. Mullins KJ, Saacke RG. Study of the functional anatomy of bovine cervical mucosa with special reference to mucus secretion and sperm transport. Anat Rec 1989; 225: 106–117.
5. 5. Liu DY, Baker HW. Morphology of spermatozoa bound to the zona pellucida of human oocytes that failed to fertilize in vitro. J Reprod Fertil 1992; 94: 71–84.
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