Coinheritance of hereditary spherocytosis with haemochromatosis: next-generation sequencing reveals

Author:

Soldin Inês HilárioORCID,Ferro Ana,Eremina Yuliana O,Bibi Mário Sérgio Nascimento

Abstract

We report the case of a man in his 50s with extravascular haemolysis, fluctuating indirect hyperbilirubinaemia, elevated transferrin saturation with hyperferritinaemia and normal liver enzymes. Spherocytes were detected in a blood smear and a mutation of unknown significance, c.1626+1G>A p.?, in intron 13 of theSLC4A1gene, was identified by next-generation sequencing (NGS). The same mutation was found in his daughter, who presented with similar laboratory changes, confirming the diagnosis of hereditary spherocytosis. Abdominal MRI showed hepatosplenomegaly with hepatic iron overload. In this context of haemolysis (without anaemia) and iron overload, a diagnosis of haemochromatosis was presumed. NGS confirmed the presence of the variants p.(His63Asp) and p.(Cys282Tyr) in heterozygosity in theHFEgene. We report this case for the rarity of co-existing two haematological diseases counteracting each other.

Publisher

BMJ

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