Note on preserving a standard suspension of live vibrios.

Author:

Ghosh H K,Lumsden W H,Hardy G J

Publisher

BMJ

Subject

General Medicine,Pathology and Forensic Medicine

Reference10 articles.

1. Practical Haematology;Dacie, J.V.,1950

2. Johnson, W. C., and Helwig, E. B. (1963).

3. Staining Methods, Histologic and Histochemical;McManus, J.F.A.; Mowry, R.W.,1960

4. PREPARATION OF A STABILATE The contents of an ampoule were cultured as above. Portions of five to six colonies were streaked on a nutrient agar slope and incubated overnight. The growth was suspended in 2 ml. of broth, and divided in two portions. To one part was added sterile glycerol (7 5 % v/v), the second portion received no preservative. In early experiments sterile Analar grade dimethylsulphoxide was added to a third portion to a final concentration of 10% (v/v). The suspensions were filled into capillary lymph tubes and then frozen and stored in solid carbon dioxide (-79°C.) as described by Cunningham, Lumsden, and Webber,1963

5. except that the inoculum (500 colonyforming units of V. cholerae 12R) was injected into the duodenum exposed through a right subcostal incision. One animal was killed with ether when it developed diarrhoea 20 hours later. The watery fluid in its caecum was mixed with an equal volume of normal rabbit serum. The mixture was frozen after adding glycerol;Feeley,1964

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1. Preservation of Vibrio cholerae by Suspension in Normal Saline;Indian Journal of Medical Microbiology;2008-04

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