1. Practical Haematology;Dacie, J.V.,1950
2. Johnson, W. C., and Helwig, E. B. (1963).
3. Staining Methods, Histologic and Histochemical;McManus, J.F.A.; Mowry, R.W.,1960
4. PREPARATION OF A STABILATE The contents of an ampoule were cultured as above. Portions of five to six colonies were streaked on a nutrient agar slope and incubated overnight. The growth was suspended in 2 ml. of broth, and divided in two portions. To one part was added sterile glycerol (7 5 % v/v), the second portion received no preservative. In early experiments sterile Analar grade dimethylsulphoxide was added to a third portion to a final concentration of 10% (v/v). The suspensions were filled into capillary lymph tubes and then frozen and stored in solid carbon dioxide (-79°C.) as described by Cunningham, Lumsden, and Webber,1963