Single-tube nested PCR in the diagnosis of tuberculosis.

Author:

Chan C M,Yuen K Y,Chan K S,Yam W C,Yim K H,Ng W F,Ng M H

Publisher

BMJ

Subject

General Medicine,Pathology and Forensic Medicine

Reference19 articles.

1. Comparison of a radiometric method (BACTEC) and Thus, the sensitivity of PCR was comparable with culture although only 12-5 pd and 100 id, respectively, of the sediment were used for PCR and culture. This was not the case in conventional culture media for recovery of mycobacteria from smear-negative specimens;Morgan, M.A.; Horstmeier, C.D.; DeYoung, D.R.; Roberts, G.D.;J Clin Microbiol,1983

2. Detection of mycobacterial antigen in cerebrospinal fluid of patients with tuberculous meningitis by enzyme extrapulmonary specimens, which did not undergo decontamination and of which the volume of sediment that could be used for culture often noticeably higher than that used for PCR, greatly enhancing the apparent sensitivity of culture compared with PCR in these specilinked immunosorbent assay;Sada, E.; Ruiz-Palacios, G.M.; Lopez-Vidal, Y.; Ponce-de-Leon, S.;Lancet,1983

3. American Thoracic Society and Centers for Disease Control. The tuberculin skin test;Am Rev Respir Dis,1981

4. The humoral immune response in tuberculosis: mens. It is not possible to increase the volume of sediment used for PCR further because the its nature, biological role, and diagnostic usefulness;Grange, J.M.;Adv Tuberc Res,1984

5. Early diagnosis of tuberproblem of inhibitors would then be insurmountable. Moreover, the sensitivity of culculosis meningitis by detection of anti-BCG secreting cells in cerebrospinal fluid;Lu, C.Z.; Qiao, J.; Shen, T.; Link, H.;Lancet,1990

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