Heterogeneity of antimitochondrial antibodies with the M2-M4 pattern by immunofluorescence as assessed by Western immunoblotting and enzyme linked immunosorbent assay.

Author:

Fusconi M,Ghadiminejad I,Bianchi F B,Baum H,Bottazzo G F,Pisi E

Publisher

BMJ

Subject

Gastroenterology

Reference29 articles.

1. Considering the anti-mitochondrial antibodies from primary biliary cirrhosis cases, 84% recognised the 74 kD protein (often in association with the 52 kD band), while the remaining 16% were negative for the 74 kD band but invariably reacted with the 52 kD one. In the ELISA test the intensity of the reactions correlated with all of the bands detected by immunoblotting except for that of 52 kD. Moreover, the correlation between the ELISA results and the immunofluorescence titres significantly increases (from p

2. immunofluorescence antimitochondrial antibody Lancet,1970

3. positive sera are different from;Walker, J.G.; Doniach, D.; Roitt, I.M.; Sherlock, S.;Lancet; Serowhich are immunoblotting reactive. The negativity of this test could be caused by a denaturing effect of the immunoblotting procedure on the antigen(s). The clinical significance of antimitochondrial antibody positivity in patients without evidence of a liver disorder'3 '4,1965

4. Heterogeneity of antimitochondrial antibodies: characterization and separation of the antigen associated with the pseudolupus erythematosus syndrome. Clin Exp Immunol;Sayers, T.Y.; Binder, T.; Berg, P.A.,1979

5. antibodies. Lancet 1980; ii: 1329-32.

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