AB0853 The role of IL-17, IL-23 serum levels in patients with ankylosing spondylitis with inflammatory bowel diseases

Abstract

BackgroundCurrently, there is a lot of interest in the frequency and risks of developing of inflammatory bowel diseases (IBD) in patients with ankylosing spondylitis (AS) [1]. IL-17 and IL-23 are one of the key pathogenetic markers of AS [2]. A lot of studies also showed that the average number of IL-17 cells was significantly increased in active Ulcerative colitis (UC) and Crohn disease (CD) patients [3]. According to some studies cytokines, such as IL-17 and IL-23, play crucial role in intestinal protection and homeostasis [4].ObjectivesThe aim of this study was to evaluate IL-17 and IL-23 serum levels in patients with AS without IBD and with AS and IBD.MethodsIn the analysis were included 50 patients with AS, fulfilling the modified New York criteria, among them man-36 (72%), woman-14 (28%), mean age of patients was 42.5±9.9, mean disease duration – 13.4±8.7 years. All patients were examined with ESR, CRP, esophagogastroduodenoscopy, colonoscopy, IL-17 and IL-23, using enzyme-linked immunosorbent assay for the quantitative measurement of IL-17 and IL-23 in serum ELISA kit. IL-17 and IL-23 levels were also studied in control group (13 healthy donors), mean IL-17 was 1.16±1.02 pg/mL, mean IL-23 was 33.03±18.02 pg/mL.ResultsAll patients had a high disease activity, mean BASDAI was 5.3± 1.8, mean ASDAS CRP 3.7±1.01, mean ASDAS ESR 3.6±1.01. IBD were diagnosed in 11 cases: 6 patients (12%) with CD and 5 patients (10%) - UC, in the remaining cases (78%-39 patients) was no intestinal pathology. Patiens with AS had higher levels of IL-17(10.4±9.1 pg/ml) and IL-23(188±156 pg/ml), than the group of healthy donors. Patients with AS and IBD had slightly lower IL-17(6.7±4.5 pg/ml) and IL-23(155.5±97 pg/ml) levels than patients with AS without IBD(p=0.03). IL-23 was positively correlated with ESR (r=0.5) and CRP (r=0.5) (p <0.001) levels, however IL-17 level had negative correlation with ESR and CRP level (r=-0.2) (p<0.001).ConclusionIL-23 and IL 17 serum levels in patients with AS and IBD was lower than in patients with AS without IBD. There is a significant association between the CRP, ESR and IL-23 level, but not with IL-17.References[1]Lukina G.V., Kulakova P.I., Novikov A.A., Savenkova N.A., Alexandrova E.A., Volnukhin E.V., Kovshik A.N. Frequency of inflammatory bowel diseases in patients with ankylosing spondylitis. Medical alphabet. 2019;2(37):12-15. https://doi.org/10.33667/2078-5631-2019-2-37(412)-12-15.[2]Sherlock JP, Taylor PC, Buckley CD. The biology of IL-23 and IL-17 and their therapeutic targeting in rheumatic diseases. Curr Opin Rheumatol. 2015 Jan;27(1):71-5.doi:10.1097/BOR.0000000000000132.[3]Fujino S, Andoh A, Bamba S, et al. Increased expression of interleukin 17 in inflammatory bowel diseases. Gut.2003;52:65-70.[4]Shen W, Durum SK. Synergy of IL-23 and Th17 cytokines: new light on inflammatory bowel disease. Neurochem Res. 2010 Jun;35(6):940-6. doi: 10.1007/s11064-009-0091-9Disclosure of InterestsNone declared