The using of gamma-octanolactone in the broiler diet: intestinal microbiome

Abstract

It is known that the inclusion of antibiotics in chicken feed leads to the development of resistance in bacteria. It is for this reason that the search for additives to alternative antibiotics becomes relevant. The ability of gamma-glucanolactone to inhibit various quorum systems (QS) LuxI/LuxR in bacteria has been demonstrated. Based on this, it can be assumed that the use of this substance in the diet will reduce infections in the intestines of poultry and improve the effects of broiler rearing. The aim of this work was to evaluate the microbiome of the iliac intestine of broilers when introducing gammaoctanolactone into the diet.The study was conducted using the sequencing method. Total DNA from samples of intestinal contents was isolated using the FastDNA ® Faecal Extraction Kit. Pair-end sequencing of DNA library amplicons was performed on the Illumina MiSeq Reagent Kit platform using the MiSeq v.2 Reagent Kit (500 cycles) (Illumina, San Diego, California, USA). The inclusion of gamma-octalactone in the diet of broilers at a dosage of 0.05 and 0,20 ml / kg w.m. / day contributes to an increase in the number of representatives of the phylum Firmicutes in the iliac intestine of poultry. The dosage of 0,05 ml / kg w.m. / day of gamma-octanolactone reduced the content of the genus Streptococcus by 4,21%; the dosage of 0,20 ml / kg w.m./ day of gammaoctanolactone increased by 10% representatives of the genera Lactobacillus and Turicibacter, relative to the control.