Affiliation:
1. Tatar Scientific Research Institute of Agriculture “Kazan Scientific Center of Russia Academy of Sciences”
2. Selection and Seed Center structural subdivision of FRC Kazan Scientific Center of RAS
Abstract
The study was conducted in order to study the polymorphism of the osteopontin gene (OPN) and to evaluate the indicators of milk productivity of Holstein first-calf cows with different genotypes. The work was carried out in the agricultural complex "Plemzavod im. Lenin" of the Atninsky district of the Republic of Tatarstan and the laboratory of the Department of Physiology, Biochemistry, Genetics and Animal Nutrition of the TatNIISKh FIT KAZNTS RAS. Genotyping was carried out by PCR-PDRF at the locus of the OPN-Bse1 I gene. The analysis of the genetic structure showed that the studied population is polymorphic by the OPN gene. The distribution of alleles and genotypes was as follows: C-0.432 and T0.568; CC – 14.3% (37 goals), CT - 57.8% (149 goals) and TT - 27.9% (72 goals). The highest milk yield for 305 days of lactation and a high yield of milk protein was observed in individuals with the CC genotype. During DNA testing of 258 Holstein firstcalf cows by PCR-PDRF of osteopontin gene polymorphism (OPN), it was revealed that the studied cattle population is polymorphic and is represented by all alleles and genotypes. According to the content of the mass fraction of fat and the yield of milk fat, a statistically significant superiority is observed in animals with a heterozygous CT genotype. It has been established that the C allele has a positive effect on the signs of milk productivity and the qualitative composition of milk.
Publisher
Saint-Petersburg State University of Veterinary Medicine
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