Results of bacteriological tests of rainbow trout (<I>Onchorinchus mykiss</I>) bioassaywhen using the grug «SmartBiotic»-Reference-Cited by-同舟云学术

Results of bacteriological tests of rainbow trout (<I>Onchorinchus mykiss</I>) bioassaywhen using the grug «SmartBiotic»

Published:2023-01-09 Issue:4 Volume: Page:161-164
ISSN:2782-6252
Container-title:Legal regulation in veterinary medicine
language:
Short-container-title:jour

Author:

Karpenko L. Yu.¹^ORCID,Bakhta A. A.¹^ORCID,Ivanova K. P.¹,Polistovskaya P. A.¹,Orlova D. A.¹^ORCID,Kalyuzhnaya T. V.¹^ORCID

Affiliation:

1. St. Petersburg State University of Veterinary Medicine

Abstract

Fish and fish products are perishable products. Therefore, if the conditions of cultivation, preparation, storage and transportation are not observed, the risk of contamination of fish with microorganisms increases. In case of doubt about the degree of freshness of fish according to organoleptic indicators, laboratory tests are carried out, one of which is bacterioscopy of smears-prints of bioassays, used in cases of suspected contamination of fish with microorganisms. The evaluation of the results of the bacteriological study is carried out by the intensity of coloring and the number of cocci, sticks in the prepared smears-prints.Bacteriological tests of smears-prints of fish muscle tissue was carried out on the basis of the laboratory of the Department of Veterinary and Sanitary Examination of the Federal State Budgetary Educational Institution "SPbGUVM" according to generally accepted methods in accordance with the "On the safety of fish and fish products". The essence of bacteriological research lies in the fact that two smears are made on the slides, the preparations are dried in the air, fixed with a triple holding over the flame of an alcohol lamp and stained by Gramm. They view several fields of vision and calculate the arithmetic mean number of microbes in one field of view.

Publisher

Saint-Petersburg State University of Veterinary Medicine

Reference12 articles.

1. Kinarevskaya, K.P. Specific features of the level of bactericidal activity of animal blood serum during pregnancy / K.P. Kinarevskaya, P.A. Polistovskaya // Knowledge of the young for the development of veterinary medicine and the agro-industrial complex of the country: materials of the international scientific conference of students, graduate students and young scientists, St. Petersburg, November 22 –23, 2018. - St. Petersburg: St. Petersburg State Academy of Veterinary Medicine, 2018. - S. 103-104.

2. Desquamation of Intestinal Epithelium as Indicator of Toxicosis in Fish / P. A. Polistovskaya, L. Yu. Karpenko, A. A. Bakhta [et al.] // International scientific and practical conference "Agro-SMART - Smart solutions for agriculture" (Agro-SMART 2018), Tyumen, July 16–20, 2018. - Tyumen: Atlantis Press, 2018. - P. 569-573.

3. Clinical and hematological picture in enteritis in calves / V. A. Trushkin, S. V. Vasilyeva, G. S. Nikitin [et al.] // Issues of legal regulation in veterinary medicine. - 2016. - No. 4. - S. 101-103.

4. Trushkin, V. A. Clinical and biochemical substantiation of the use of the probiotic "Avena" in enteritis in calves: specialty 06.02.01 "Diagnosis of diseases and therapy of animals, pathology, oncology and morphology of animals": dissertation for the degree of candidate of veterinary sciences / Trushkin Vyacheslav Alexandrovich. - St. Petersburg, 2011. - 156 p.

5. Vasilyeva S.V., Pilaeva N.V., Trushkin V.A. [et al.] Dynamics of enzymatic activity of quail blood serum when using various feed additives // Questions of legal regulation in veterinary medicine. - 2015. - No. 3. - P. 235-237.