Analysis of the intestinal microbiome in young laying hens of the "Hi-Line" cross against the background of the use of the mineral shungite

Abstract

Growing young chickens under conditions of intensive keeping technology can lead to a decrease in nonspecific resistance, the manifestation of immunodeficiencies and increased susceptibility of the bird to adverse environmental factors and various opportunistic microorganisms. The presence of mycotoxins in feed is one of the main feed stresses. At the same time, stresses associated with vaccinations are added in the future and, of course, heat stresses play a special role in the summer months. The purpose of the work is to study the influence of the domestic natural adaptogen from the mineral shungite on productivity indicators and on the composition of the intestinal microbiome in young chickens of egg breed. The object of research was young laying hens of the High Line cross. To conduct the experiment at a day old, 3 groups of young animals were formed (1 control and 2 experimental - with different concentrations of the mineral shungite). The raw material for the production of a mineral additive from shungite was shungite from the Zazhogino deposit (Republic of Karelia, Medvezhyegorsk region). Fraction 0.2-0.8 mm, humidity up to 10%. Carbon content (C) 25-35%. The composition of the compound feed formula was developed in order to increase the immune status of chickens, accelerate the development of the internal organs of chickens in the early period and, as a result, increase the safety, uniformity and business yield of pullets. The intestinal microbiome of young laying hens was determined using high throughput sequencing. It should be noted that among the bacteria inhabiting the gastrointestinal tract, the function of digestion is performed by several phyla, the main part of which are bacteria of the phyla Actinobacteria, Firmicutes, Bacteroidetes and Proteobacteria. Thus, based on the results of high-throughput sequencing, the corrective effect of the adaptogen from shungite at different concentrations on the composition of the bird's intestinal microflora was established. The positive effect of shungite at a concentration of 1.0 kg/t of feed on the increase in the number of normoflora of blind processes was confirmed. A significant effect of the additive from the mineral shungite on the number of cellulolytic bacteria was revealed.