The efficacy of various protocols of colloidal centrifugation of bovine sperm for IVF

Author:

Nikitin G. S.1ORCID,Achilov V. V.1ORCID,Mirzakaeva I. I.1

Affiliation:

1. St. Petersburg State University of Veterinary Medicine

Abstract

The article presents data on the effectiveness of using various sperm preparation protocols by colloidal centrifugation (density gradient centrifugation method) before procedures of assisted reproductive technologies, including in vitro artificial insemination. The use of the method can affect the quality of sexual gametes and the viability of embryos, increase the percentage of successful embryotransplantation and contribute to the appearance of highly productive offspring.As a result of the research, the most optimal protocol of centrifugation by the method of gradient layering with indicators of the first and second centrifugation 700g – 20 min and 100g – 10 min, respectively, was determined by morphofunctional characteristics. The average rate of motile spermatozoa was 84.1±5.1%, progressive spermatozoa – 68.7±5.1%, the rate of rectilinear translational motion was 49.69 microns/sec. Morphology and viability meet quality standards.

Publisher

Saint-Petersburg State University of Veterinary Medicine

Reference9 articles.

1. GOST 32277 – 2013. Means of reproduction. Sperm. Methods of testing physical properties and biological, biochemical, morphological analyses. – M.: Publishing House Standartinform, 2014. – 16p.

2. Andreev, G.M. The main causes of violations of reproduction of cattle. Methodical manual / G.M. Andreev, V.U. Davydov, K.V. Plemyashov. – St. Petersburg: Publishing house of FGBOU SPbGAVM, 2004. – 13p.

3. Zinovieva, N.A. Assisted reproductive technologies: the history of formation and the role in the development of genetic technologies in cattle breeding (review) / N.A. Zinovieva, S.V. Pozyabin, R.Yu. Chinarov // Agricultural biology, Agricultural biology. – 2020. – No. 2. – P. 225-242.

4. Nikitin, G.S. The use of correlation analysis to determine the direction and quantitative measurement of connections in biometrics (on the example of zoohygienic assessment of feeding broiler chickens with various feeds) / G.S. Nikitin, M.G. Nikitina // The practice of using natural science methods in applied socio-humanitarian research. Collection of materials of the methodological seminar. – Tolyatti.: Tolyatti State University Publishing House. – 2014.

5. Nikitin, G.S. Modern approaches to obtaining and cryopreservation of cattle embryos in vitro / G.S. Nikitin // International Bulletin of Veterinary Medicine. - 2021. – No.3. – P. 192-205.

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3