Increasing the viability of rooster sperm during cryopreservation through the use of enzymatic antioxidants

Abstract

Cryopreservation of rooster semen has found wide application in creation of reproductive cell’s cryobanks. Compare to other farm animal’s semen, quality of frozen/thawed bird semen are often lower. This factor makes choice of individual ejaculates for the purposes of cryopreservation more difficult. In our study, we considered the possibility of improving frozen/thawed semen performance by adding enzymatic antioxidants to diluents. It has been shown that during the vitrification of reproductive cells, the addition of exogenous enzymatic antioxidants reduces the destructive effect of reactive oxygen species, which indicates the possibility of improving method by reducing oxidative stress to cells. When added to the diluent for cryopreservation rooster’s sperm LKS-1 superoxide dismutase in amount of 75 IU cell viability increased by 3,65 %, when was added catalase in amount of 200 μg/ml, cell viability increased by 5,27 %.