Polyphenols Analysis by LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, Antimicrobial Activities of <em>Jatropha multifida</em> L. Extracts Used in Benin Pharmacopoeia

Author:

Dah-Nouvlessounon DurandORCID,Chokki MichaelleORCID,Agossou Essé A,Houédanou Jean-Baptiste,Nounagnon Martial,Sina Haziz,Vulturar RomanaORCID,Heghes Simona CodrutaORCID,Cozma Angela,Mavoungou Jacques François,Fodor AdrianaORCID,Baba-Moussa Farid,Suharoschi RamonaORCID,Baba-Moussa LamineORCID

Abstract

Jatropha multifida L. is a plant belong to Euphorbiaceae family used in Benin pharmacopoeia because of its medicinal properties. The objective of this work is to appreciate the therapeutic power of this plant through the evaluation of some of its biological activities. After the preliminary phytochemistry, the polyphenols and flavonoids were quantified and identified by the LC-MS-ESI. The antimicrobial power of the extracts was investigated by agar medium diffusion. The antioxidant power of the extracts was evaluated by the reduction of the DPPH radical, the ABTS radical cation, the feric ion (FRAP) and the lipid peroxidation (LPO). Anti-inflammatory activity was assessed by inhibition of protein (albumin) denaturation. Indeed, different phenolic and flavonoid compounds namely, 2-Hydroxybenzoic acid, o-Coumaroylquinic acid, Apigenin-apiosyl-glucoside, Luteolin-galactoside, Luteolin-glucoside, Luteolin-rhamnoside, Quercetin-glucoside, Quercetin-arabinoside, Dicaffeoyquinic acid, Kaempferol-rhamnoside were identify. The J. mutifida extracts has a bactericidal effect with reference strains of wich CMBs vary from 22.67 mg/ml (S. aureus, S. enteridis, L. monocytogenes and C. albicans) to 47.61 mg/ml (E. coli) and with the meat isolated strains (S. equorum, S. saprophiticus, S. haemoliticus, S. cohnii and S. lentus). Ethanolic extract show the highest DPPH radical scavenging activity (IC50 = 0.72&plusmn;0.03 mg/ml) while methanolic extract showed the highest ferric ion reduction (46.23&plusmn;1.10 &micro;gEAA/g). Contrary to the FRAP method, the reducing power with ABTS method of water-ethanolic extract was greater (0.49&plusmn;0.11 mol ET/g). The same extract show the highest albumin denaturation inhibition power (97.31&plusmn;0.35%) at 1000&micro;g/ml. J. multifida extracts are rich in bioactive compound with good biological activity

Publisher

MDPI AG

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