Physiological features of semen resistance of stallions to cooling with different antigenic profile of erythrocytes

Abstract

The article shows the physiological features of the survival ability of stallion semen at a temperature of 2 to 5°C with different antigenic features according to blood group systems. It was shown that in the presence of the antigens cgm/ceg, ad/dk, cgm/dk, cgm/d, dg/di on D system erythrocytes, the semen survival ability at 2 to 5°C was observed for a maximum of 50 hours. The carriers of the antigens cegm/cgm, ad/de, cgm/cgm, аd/bcm, bcm/dg, ad/d, cegm/dg, bcm/cgm, bcm/de, cegm/d, de/cgm, cegm/dk, dk/d, de/d, de/dk, cgm/dg, dk/de antigens have obtained a medium sperm resistance to cooling with a semen survival time of 50 to 75 hours. Stallions with the antigens bcm/d, bcm/dk, ad/cgm, dg/dk, cgm/de, dg/cgm, dk/dk of the D blood group showed a high semen resistance to cooling at 2 to 5°C in the form of a survival time of more than 75 hours. When the experimental stallions inherited the a/- antigen of the K system, the semen survival ability was increased by 7.24 h (P < 0.05) and the absolute survival ability by 24.18 standard units (P < 0.05) compared to the absence of this system antigen. The absence of -/- erythrocytic antigens of the C blood group is associated with an increase (P < 0.05) of 10.21 h in the survival ability and 30.29 standard units in the absolute semen survival ability compared to ejaculates from stallions with the a/- antigen of this erythrocytic system. The absence of the -/- antigen of the A system in stallions and the presence of the а/- antigen are followed by an increase in ejaculate resistance to cooling by 14 h (Р < 0,05) as compared to the control. At the same time, the absolute semen survival ability is 38.69 and 45.94 standard units higher than the control values (P < 0.01). Inheritance by stallions of the ad/- antigen by stallions was followed by a higher semen resistance to cooling as compared to the control by only 9.63 h (Р < 0.05) and a lower resistance compared to а/- and -/- by 5 h. The practical application of the data obtained is that it becomes possible to predict the shelf life of freshly dissolved cooled semen before artificial insemination, which is especially important for long transport of semen doses.