DNA methylation analysis: speedup of bisulfite-mediated deamination of cytosine in the genomic sequencing procedure.
Author:
Affiliation:
1. Shujitsu University, School of Pharmacy
2. Okayama University Advanced Science Research Center, Department of Genomics and Proteomics
3. National Cancer Center Research Institute, DNA Methylation and Genome Function Project
Publisher
Japan Academy
Subject
General Physics and Astronomy,General Agricultural and Biological Sciences,General Medicine
Link
http://www.jstage.jst.go.jp/article/pjab/80/4/80_4_189/_pdf
Reference11 articles.
1. Reactions of Uracil and Cytosine Derivatives with Sodium Bisulfite
2. Addition of sodium bisulfite to uracil and to cytosine
3. Reaction of sodium bisulfite with uracil, cytosine, and their derivatives
4. Bisulfite Modification of Nucleic Acids and their Constituents
5. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.
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2. Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA;Clinical Epigenetics;2021-10-18
3. In Response to Abiotic Stress, DNA Methylation Confers EpiGenetic Changes in Plants;Plants;2021-05-30
4. Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data;Genome Biology;2018-03-15
5. An optimized rapid bisulfite conversion method with high recovery of cell-free DNA;BMC Molecular Biology;2017-12
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