Affiliation:
1. Samara State Medical University
2. Samara National Research University named after academician S.P. Korolev (Samara University)
Abstract
Background. Contemporary dental diagnosis should supplement clinical examination with instrumental diagnostic techniques. Raman spectroscopy has become widely adopted due to high spatial resolution, non-invasiveness, the lack of strict requirements and ease of sample preparation.Objectives. A qualitative assessment of enamel mineral and organic composition dynamics using Raman spectroscopy.Methods. Raman spectroscopy was used as primary research method. The trial bench consisted of a Shamrocksr-303i high-resolution digital spectrometer with built-in DV420A-OE cooled optical detector (spectral range 200–1200 nm), RPB-785 optic fibre scattering probe integrated with LuxxMaster LML-785.0RB-04 laser source with wavelength 785 nm.Software spectrum processing was performed with Wolfram Mathematica 9. Spectra were denoised with a median filter (5 points), the approximating line (fifth order polynomial) of autofluorescent component was determined in 700–2200 cm-1 range using an iterative algorithm and then subtracted to receive isolated Raman spectra.Linear discriminant analysis (LDA) of data was performed with the IBM SPSS Statistics package. Results. The assay included 28 teeth, with 14 extracted for orthodontic indications and 14 — for chronic periodontitis. Spectral dental enamel dynamics has been established in periodontal disease and after the in-office bleaching procedure. The evidence obtained can be applicable in dental practice to verify patients at risk of periodontal disease by interpreting the tooth enamel spectral properties, as well as prior to in-office bleaching.Conclusion. We demonstrate high efficiency of Raman spectroscopy for qualitative assessment of the mineral and organic enamel composition dynamics in various settings. Raman spectroscopy is confirmed effective and versatile in various aetiologies. It was successfully applied to diagnose periodontitis by changes in the organic and mineral enamel composition and evaluate enamel after in-office bleaching.
Publisher
Kuban State Medical University
Reference15 articles.
1. Ziulkina L., Frolova K., Efremova A., Akimova S. Current state of the issue of diagnosis of major dental diseases. Sovremennaya Nauka: Aktual’nye ProblemyTeorii i Praktiki. Seriya: Estestvennye i Tekhnicheskie Nauki. 2020; 6: 188–193 (In Russ., English abstract). DOI: 10.37882/2223-2966.2020.06.13
2. Magsumova O.A., Tkach T.M., Postnikov M.A., Ryskina E.A., Korchagina M.S., Dudina S.E., Polkanova V.A., Odnoletkova I.I. Etiopathogenetic aspects of the occurrence of dental discolouration. Clinical Dentistry (Russia). 2021; 1(97): 22–29 (In Russ., English abstract). DOI: 10.37988/1811-153X_2021_1_22
3. Gonchukov S., Sukhinina A., Bakhmutov D., Minaeva S. Raman spectroscopy of saliva as a perspective method for periodontitis diagnostics. Laser Physics Letters. 2012; 9(1): 73–77. DOI: 10.1002/lapl.201110095
4. Minaeva S.A., Mikhaĭlovskiĭ A.A., Bukharova T.B., Antonov E.N., Gol’dshteĭn D.V., Popov V.K., Volkov A.V. The morphological investigation of the hard tissues of the facial skeleton with the use of combined scattering spectroscopy. Rossiiskaya Stomatologiya. 2015; 8(1): 3–10 (In Russ., English abstract). DOI: 10.17116/rosstomat2015813-10
5. Gatin E., Nagy P., Paun I., Dubok O., Bucur V., Windisch P. Raman Spectroscopy: Application in Periodontal and Oral Regenerative Surgery for Bone Evaluation. IRBM. 2019; 40(5): 279–285. DOI: 10.1016/j.irbm.2019.05.002
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献