Affiliation:
1. Privolzhsky Research Medical University;
Kirov State Medical University
2. Kirov Research Institute of Hematology and Blood Transfusion under the Federal Medical Biological Agency
3. Kuban State Medical University
Abstract
Aim. Experimental evaluation of the effect of some cryoprotectors on crystallogenic properties of mice blood serum at intraperitoneal administration.Material and methods. The experiment was performed on 40 adult white line mice divided into 4 equal groups. Animals from the control (fi rst) group received a single intraperitoneal injection of saline (0.5 ml agent/animal), animals of the second-fourth groups — 3 % solution of glycerin, dimethyl sulphoxide and trombocryodmac (N, N-dimethylacetamide), respectively, in the same quantity.Results. It is shown that single intraperitoneal introduction of cryoprotectants (solutions of glycerin, dimethyl sulphoxide and trombocryodmac) has a systemic effect on the mouse organism, which is manifested through the changes of crystallogenic properties of blood serum. 3% solution of glycerin, dimethyl sulphoxide and trombocryodmac have multidirectional infl uence on crystallogenic properties of mouse blood serum. The most pronounced physiological effect was established for dimethyl sulphoxide.Conclusion. Our studies demonstrate the presence of systemic effects in cryopreservative substances. It was confi rmed by the changes of crystallogenic properties of mice blood serum under intraperitoneal administration of cryopreservatives.
Publisher
Kuban State Medical University
Reference12 articles.
1. Bizjak D.A., Jungen P., Bloch W., Grau M. Cryopreservation of red blood cells: Effect on rheologic properties and associated metabolic and nitric oxide related parameters. Cryobiology. 2018; 84: 59–68. DOI: 10.1016/j.cryobiol.2018.08.001
2. Kostyaev A.A., Martusevich A.K., Andreev A.A. Toxicity of cryoprotectants and cryoconservants on their basis for blood components and bone marrow (review article). Nauchnoe Obozrenie. Meditsinskie Nauki. 2016; 6: 54–74 (In Russ., English abstract).
3. Ladeira C., Koppen G., Scavone F., Giovannelli L. The comet assay for human biomonitoring: Effect of cryopreservation on DNA damage in different blood cell preparations. Mutat. Res. 2019; 843: 11–17. DOI: 10.1016/j.mrgentox.2019.02.002
4. Liu J., Ding W., Zhou X., Kang Y., Zou L., Li C., Zhu X., Gao D. Deglycerolization of red blood cells: A new dilution-fi ltration system. Cryobiology. 2018; 81: 160–167. DOI: 10.1016/j.cryobiol.2018.01.006
5. Zaitseva O.O., Polezhaeva T.V., Svedentsov E.P., Solomina O.N., Utemov S.V. Effi ciency of application original preservatives for conservation leukocytes at -40°C. Zhurnal Stress-Fiziologii i Biokhimii. 2011; 7(4): 197–206 (In Russ., English abstract).