Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation-Reference-Cited by-同舟云学术

Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Published:2010-06-20 Issue:3 Volume:22 Page:371-379
ISSN:1883-8049
Container-title:Journal of Robotics and Mechatronics
language:en
Short-container-title:J. Robot. Mechatron.

Author:

Yamanishi Yoko, ,Sakuma Shinya,Iyanagi Tomohiro,Arai Fumihito,Arai Tatsuo,Hasegawa Akiyuki,Tanikawa Tamio,Ichikawa Akihiko,Satoh Osamu,Nakayama Akihiro,Aso Hiroshi,Goto Mitsuhiro,Takahashi Seiya,Matsukawa Kazutsugu, , , , , , ,

Abstract

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-in-one unified microfluidic chip was successfully designed and fabricated.

Publisher

Fuji Technology Press Ltd.

Subject

Electrical and Electronic Engineering,General Computer Science

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