Local Ablation of a Single Cell Using Micro/Nano Bubbles

Abstract

We have successfully produced mono-dispersed microgas bubbles less than around 10 µm in diameter in an electrically induced ultrasonic field. The discharged output power and conductive area of the micro-electrode are controlled by glass shell insulation around the copper micro-wire. A small space between the wire and glass tip, a “bubble reservoir,” contributes to the stabilization of the electric discharge and directional bubble generation. The directionally dispensed bubbles can be used for processing soft materials such as biological cells. For the present study, the cell membrane has successfully been processed with resolution of a few µm order and without any thermal collateral damage.