Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells-Reference-Cited by-同舟云学术

Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells

Published:2006-12-20 Issue:6 Volume:18 Page:827-830
ISSN:1883-8049
Container-title:Journal of Robotics and Mechatronics
language:en
Short-container-title:J. Robot. Mechatron.

Author:

Inoue Yusuke, ,Ishimaru Ichirou,Yasokawa Toshiki,Ishizaki Katsumi,Yoshida Makoto,

Abstract

We proposed using spectroscopy-tomography of single cells as a diagnostic tool for improving early cancer detection and treatment. This technology potentially obtains the 3D distribution of cellular components at high spatial resolution. We used spectroscopy-tomography to generate and analyze a series of cross-sectional images of a borosilicate glass microsphere 10µm in diameter, easily determining the distribution of internal submicron defects in the microsphere. We also used this to estimate the 3-dimensional refractive index distribution in a single human breast cancer cell. The nucleus, which has a high refractive index, is clearly distinguishable.

Publisher

Fuji Technology Press Ltd.

Subject

Electrical and Electronic Engineering,General Computer Science

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