First Report of Tomato severe rugose virus in Chili Pepper in Brazil

Abstract

Three definitive and three tentative begomovirus species have been reported in tomato fields in Brazil according to a recent review (1). Extensive surveys have been conducted since the 1990s in solanaceous weeds and other crops planted close to tomato fields, but no tomato-infecting geminiviruses have been reported on those crops. During November 2003, leaves of one chili pepper plant “dedo-de-moça” (Capsicum baccatum var. pendulum) showing symptoms of yellow mosaic and leaf distortion were collected in Petrolina de Goiás (Goiás State). Serological analyses were carried out with polyclonal antisera produced in our laboratory against the following viruses: Potato virus Y (PVY), Pepper yellow mosaic virus (PepYMV), Tomato spotted wilt virus (TSWV), Tomato chlorotic spot virus (TCSV), Groundnut ringspot virus (GRSV), and Chrysanthemum stem necrosis virus (CSNV). Serological data showed that the plant was not infected with any of these viruses. A begomovirus-specific DNA-A fragment of 1.3 kb was amplified by polymerase chain reaction (PCR) from the analyzed plant. The fragment shared 98% identity to the partial coat protein coding region (CP), 94% to the intergenic region (IR), and 95% to the partial AC1 coding region of Tomato severe rugose virus (ToSRV) (GenBank Accession No. AY029750). Total DNA from the original infected plant was used to biolistically inoculate healthy plants of C. annuum and C. baccatum var. pendulum. Four resulting symptomatic plants, two from C. annuum and two from C. baccatum, were tested using PCR for begomovirus, and the nucleotide sequence of the amplified fragment confirmed they were infected with ToSRV. Whitefly inoculation of C. annuum, C. baccatum, and tomato was also performed, and all plants expressing symptoms were confirmed to be infected with ToSRV by sequencing a begomovirus-specific amplified fragment. Cloning of the complete DNA-A was achieved by using TempliPhi (Amersham Biosciences, Piscataway, NJ) amplification and digestion with a single cutting restriction endonuclease (2). Sequencing of several clones showed that the complete DNA-A (GenBank Accession No. DQ207749) was 97% identical to ToSRV, confirming the results of the previous PCR analysis. The deduced amino acid sequences showed identities of 97% to the CP, 95% to AC1, 96% to AC2, 96% to AC3, and 88% to AC4 of ToSRV. Although begomoviruses have not yet been causing any significant losses in chili pepper in Brazil, they may be of potential importance. Moreover, chili pepper, a plant commonly found in gardens throughout the country, may serve as an alternate host in tomato-producing areas. To our knowledge, this is the first report of a begomovirus infecting chili pepper in Brazil. References: (1) C. M. Fauquet et al. Arch. Virol. 148:405, 2003. (2). A. K. Inoue-Nagata et al. J Virol Methods 116:209, 2004.