Characteristics of a Potyvirus of the Bean Yellow Mosaic Virus Subgroup in Sesbania speciosa Germ Plasm

Abstract

A plant of Sesbania speciosa with leaf mosaic and distortion symptoms was identified in a germ plasm regeneration plot at Griffin, Georgia. The Sesbania virus produced mild or moderate mosaic symptoms on Glycine max cvs. Bragg and Tracy M, Lupinus albus, Nicotiana benthamiana, Pisum sativum cv. Perfected Wales, Phaseolus vulgaris cvs. Black Turtle, Bountiful, and Pinto, and did not infect N. tabacum. Bean yellow mosaic potyvirus (BYMV) and pea mosaic potyvirus (PMV) do not infect Perfected Wales pea and they produce mosaic, distortion, and necrosis on white lupine. The PMV strain tested produced much more severe symptoms on the three green beans, with top necrosis on Pinto. BYMV produced local latent infection of N. tabacum and BYMV and PMV produced mosaic with distortion on N. benthamiana. The Sesbania virus was seed-transmitted at a low rate in S. speciosa. Indirect-enzyme-linked immunosorbent assay tests with a general potyvirus monoclonal antibody and BYMV and white lupine mosaic virus (WLMV) polyclonal antisera were strongly positive. Tests of the Sesbania virus against a monoclonal antibody panel suggests that it is not BYMV or any of the previously described subgroup members, but is a member of the BYMV subgroup. This is the first report of a seedborne BYMV-like virus of Sesbania spp.