Purification and Characterization of Peach Mosaic Virus

Author:

Gispert Carmen1,Perring Thomas M.1,Creamer Rebecca2

Affiliation:

1. Department of Entomology

2. Department of Plant Pathology, University of California, Riverside 92521

Abstract

Virus particles were observed by transmission electron microscopy in preparations extracted from symptomatic leaves of Chenopodium amaranticolor that had been mechanically inoculated with peach mosaic virus. The particles were long, flexuous, filamentous rods with an average length of 888 nm. Purified preparations had an A 260/280 nm ratio of 1.25. RNA extracted from purified virus was approximately 8.1 kilobases, and a capsid protein of approximately 27 kDa was found. Polyclonal rabbit antiserum, produced against purified virus, reacted with samples from peach mosaic and cherry mottle leaf-infected plants when used in Western blot analysis.

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

Cited by 14 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Peach latent mosaic viroid (American mosaic of peach);CABI Compendium;2022-01-07

2. Prunus persica (Peach);Encyclopedia of Plant Viruses and Viroids;2019

3. Detection and Identification of Viruses and Viroids;Microbial Plant Pathogens;2017-02-16

4. Control of Pome and Stone Fruit Virus Diseases;Advances in Virus Research;2015

5. Detection of Virus and Viroid Pathogens in Plants;Microbial Plant Pathogens-Detection and Disease Diagnosis:;2010-10-19

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