Effect of Simulated Wind-Driven Rain on Duration and Distance of Dispersal of Xanthomonas axonopodis pv. citri from Canker-Infected Citrus Trees

Abstract

Dynamics of dispersal of the bacteria that causes citrus canker (Xanthomonas axonopodis pv. citri) were assessed in simulated wind-driven rain splash. The wind/rain-splash events were simulated using electric blowers to generate turbulent wind (15 to 20 m s-1) and sprayer nozzles to produce water droplets entrained in the wind flow. The splash was blown at an inoculum source of canker-infected trees 1 m downwind. The splash downwind of the source of the infected trees was collected by vertical panel samplers and funnel samplers. The duration over which bacteria were dispersed in spray was assessed in continuous wind at intervals from 0 to 52 h after commencing the simulated rain splash event. In one experiment on 11 February 2003, a total of 1.48 × 106 bacteria were collected by panels 1 m downwind from the inoculum source during the first 10 min of dispersal, but the numbers declined to 3.60 × 105 bacteria after 1 h and ranged between 1.42 × 105 and 1.93 × 104 up to 52 h. In a more detailed study (15 July 2003) of dispersal duration over 4 h, the greatest quantity of bacteria collected by panel samplers were dispersed in the first 5-min period (1.01 × 108 bacteria collected). By 10 min after initiation of dispersal, approximately one-third (3.09 × 107 bacteria collected) of the initial number was being dispersed, and by the end of the first hour, only one-tenth (1.31 × 107 bacteria collected) of the initial quantity was dispersed. Funnel samplers placed at ground level under the trees showed a similar trend. The distance to which bacteria were dispersed in wind-blown splash was also tested under simulated conditions: on 18 September 2003, bacteria were collected by panel samplers at all distances sampled (1, 2, 4, 6, 8, 10, and 12 m) with the greatest number of bacteria deposited at 1 m (4.93 × 106 bacteria collected), while 2.22 × 103 bacteria were deposited over a 10-min period 12 m from the inoculum source. Wind speed declined from 19.5 m s-1 upwind of the trees to 2.8 m s-1 1 m downwind, and by 4 m downwind from the inoculum source, movement was similar to the surrounding air. The data on duration and distance of dispersal were best described by power law regression models compared to exponential models. Citrus canker is readily dispersed in wind-driven rain and is dispersed in large quantities immediately after the stimulus occurs, upon which wind-driven splash can disperse inoculum over a prolonged period and over a substantial distance.