Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase

Author:

Ray Suvendra K.,Rajeshwari R.,Sonti Ramesh V.

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight, a serious disease of rice. A virulence- and xylanase-deficient mutant of Xoo was isolated following ethyl methane sulfonate (EMS) mutagenesis. A cosmid clone that restored virulence and xylanase secretion was obtained from a genomic library by functional complementation. Trans-poson mutagenesis and marker exchange studies revealed genes on the cloned DNA that were required for xylanase production and virulence. Sequence analysis with trans-poson-specific primers revealed that these genes were homologues of xps F and xps D, which encode components of a protein secretion system in Xanthomonas campestris pv. campestris. Enzyme assays showed xylanase accumulation in the periplasmic space and cytoplasm of the xps F mutant and the complementing clone restored transport to the extracellular space.

Publisher

Scientific Societies

Subject

Agronomy and Crop Science,General Medicine,Physiology

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