An Oomycete-Specific Leucine-Rich Repeat-Containing Protein Is Involved in Zoospore Flagellum Development in Phytophthora sojae

Author:

Zhang Baiyu123,Zhang Zhichao123,Yong Saijiang123,Yu Shuyang123,Feng Hui123,Yin Maozhu123,Ye Wenwu123ORCID,Wang Yuanchao123,Qiu Min123ORCID

Affiliation:

1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

2. The Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

3. Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

Abstract

A leucine-rich repeat (LRR) is a widespread structural motif of 20 to 30 amino acids with characteristic repetitive sequences rich in leucine. LRR-containing proteins are critical for ligand recognition and binding, participating in plant development and defense. Like plants, oomycetes also harbor genes encoding LRR-containing proteins, but their functions remain largely unknown. We identified a zoospore-upregulated gene from Phytophthora sojae with LRRs and an extra structural maintenance of chromosomes-like domain. We generated knockout and complemented knockout strains of this LRR protein and found that its deletion resulted in a pronounced reduction in zoospore mobility and chemotaxis, cyst germination, and virulence. Interestingly, micro-examination of zoospores under a scanning electron microscope revealed irregularly shaped zoospores without flagella in these deletion mutants. In addition, the reintroduction of this LRR protein into the knockout mutant reversed all the deficiencies. Our data demonstrate a critical role for the Phytophthora LRR protein in modulating zoospore development, which impairs migration to the host soybean and affects the spread of Phytophthora pathogens.

Funder

Chinese Modern Agricultural Industry Technology System

China National Funds for Innovative Research Groups

China Postdoctoral Science Foundation

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

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