Reduction in Common Rust Severity Conferred by the Rp1D Gene in Sweet Corn Hybrids Infected by Mixtures of Rp1D-Virulent and Avirulent Puccinia sorghi

Abstract

The Rp1D gene confers a hypersensitive, chlorotic-fleck, resistant reaction to Puccinia sorghi, the casual agent of common rust of corn. About 40% of commercial sweet corn hybrids carry the Rp1D gene. Sine 1999, Rp1D-virulent (D-virulent) isolates of P. sorghi have occurred regularly in populations of P. sorghi in North America. Observations from sweet corn hybrid nurseries and other trials indicate that the frequency of D-virulent isolates affects severity of rust on Rp1D hybrids; however, the frequency of D-virulence at which the Rp1D gene is rendered completely ineffective is not known. The objective of this study was to assess whether common rust severity is reduced by the Rp1D gene in sweet corn hybrids infected by mixtures of D-virulent and Rp1D-avirulent (avirulent) P. sorghi. Forty pairs of Rp1D-resistant and susceptible (rp1d) versions of sweet corn hybrids from six different commercial breeding programs were evaluated in 2003 and 2004 in trials inoculated with one of five different ratios of avirulent:D-virulent inocula: 100:0, 90:10, 80:20, 60:40, or 0:100. When D-virulent P. sorghi was 100% of initial inoculum, common rust was equally severe on Rp1D and rp1d versions of the same hybrid. Thus, the Rp1D gene did not confer partial or residual resistance in these trials. When initial inocula consisted of 40% or less D-virulent P. sorghi, rust was significantly less severe on Rp1D versions than on rp1d versions of the same hybrids. Relationships between rust severity on Rp1D and rp1d versions of hybrids were explained by linear regressions in all trials. Slope coefficients (i.e., rust severity on Rp1D hybrids as a proportion of that on rp1d hybrids) were related to the percentage of D-virulent P. sorghi in the initial inoculum and were 0.21, 0.29, 0.51, 0.64, and 0.93 in 2003 and 0.25, 0.50, 0.67, 0.76, and 1.0 in 2004 for trials inoculated with 0, 10, 20, 40, and 100% D-virulent P. sorghi, respectively. Thus, the Rp1D gene may convey levels of control in proportion to the frequency of virulence in mixed populations of D-virulent and avirulent P. sorghi when the frequency of virulent isolates is less than 40%.