First Report of Erwinia persicina from Phaseolus vulgaris in Spain

Author:

González A. J.1,Tello J. C.2,Cara M. de2

Affiliation:

1. Laboratorio de Fitopatología, SERIDA, carretera de Oviedo s/n, 33300 Villaviciosa, Asturias. Spain

2. Departamento de Producción Vegetal. Universidad de Almería, 04120 Almería, Spain

Abstract

A previously unreported leaf spot disease of common bean, which caused losses as much as 50% of the crops, was observed in southeastern Spain (Almería, Granada, and Málaga provinces) in November 2003. In 2004, samples of cv. Donna with chlorotic and necrotic leaf spots were collected from Granada and processed for microbiological analysis. Bacteria isolated from the symptomatic leaves were determined to be fermentative on the basis of the ability to metabolize glucose in aerobic and anaerobic conditions. Three isolates were selected for pathogenicity tests. Bacterial suspensions (108 CFU/ml) were spray inoculated on bean seedlings (3 true leaves) of cv. Andecha. Beans were covered with transparent plastic bags for 2 days and held in an incubation chamber at 22°C and 80% relative humidity with a 12-h photoperiod. Assays were repeated at least twice. Symptoms that developed on plants inoculated with the three isolates were similar to those originally observed, while symptoms did not occur on control plants (inoculated with distilled water). The pathogenic isolates were identified by sequencing of the 16S rDNA after amplification (2). The amplified sequences were compared to available DNA sequences in databases by using BLAST (1); 99% homology with 16S rDNA of Erwinia persicina was shown. Microbiological characteristics (gram staining, motility, morphology, and results of biochemical tests) were in agreement with the molecular identification of the isolates. E. persicina has been isolated from bean in the United States (4) and described on tomato, banana, and cucumber in Japan (3). To our knowledge, this is the first report of E. persicina from common bean in Spain and in Europe. References: (1) S. F. Altschul et al. J. Mol. Biol. 215:403, 1990. (2) U. Edwards et al. Nucleic Acids Res. 17:7843, 1989. (3) M. V. Hao et al. Int. J. Syst. Bacteriol. 40:379, 1990. (4) M. L. Schuster et al. Fitopatol. Bras. 6:345, 1981.

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3