Genotypic and resistance profile analysis of two oat crown rust differential sets urge coordination and standardisation

Author:

Nguyen Duong T.1,Henningsen Eva Celeste23,Lewis Dave4,Mago Rohit5,McNeil Meredith6,Suchecki Radoslaw7,Boden Scott8,Sperschneider Jana4,Kianian Shahryar9,Dodds Peter4,Figueroa Melania10

Affiliation:

1. CSIRO, 2221, Agriculture and Food, Adelaide, South Australia, Australia;

2. CSIRO, 2221, Agriculture and Food, Canberra, Australian Capital Territory, Australia

3. Australian National University, 2219, Research School of Biology, Canberra, Australian Capital Territory, Australia;

4. CSIRO, 2221, Agriculture and Food, Canberra, Australian Capital Territory, Australia;

5. CSIRO, 2221, Agriculture & Food, GPO Box 1700, Canberra, Australian Capital Territory, Australia, 2601, ;

6. CSIRO, 2221, Agriculture and Food, 306 Carmody Road, St Lucia , Queensland, Australia, 4067;

7. CSIRO, 2221, Adelaide, South Australia, Australia;

8. The University of Adelaide, 1066, School of Food, Agriculture, and Wine, Adelaide, South Australia, Australia;

9. USDA-ARS Cereal Disease Laboratory, 57840, 1551 Lindig Street, Saint Paul, Minnesota, United States, 55108;

10. Commonwealth Scientific and Industrial Research Organisation, 2221, Agriculture and Food, Canberra, Australian Capital Territory, Australia;

Abstract

Puccinia coronata f. sp. avenae (Pca) is the causal agent of the disease known as crown rust which represents a bottleneck in oat production worldwide. Characterisation of pathogen populations often involves race (pathotype) assignments using differential sets, which are not uniform across countries. This study compared virulence profiles of 25 Pca isolates from Australia using two host differential sets, one from Australia and one from the USA. These differential sets were also genotyped using DArT sequencing technology. Phenotypic and genotypic discrepancies were detected on eight out of 29 common lines between the two sets, indicating that pathogen race assignments based on those lines are not comparable. To further investigate molecular markers that could assist in the stacking of rust resistance genes important for Australia, four published Pc91-linked markers were validated across the differential sets and then screened across a collection of 150 oat cultivars. Drover, Aladdin, and Volta were identified as putative carriers of the Pc91 locus. This is the first report to confirm that the cultivar ‘Volta’ carries Pc91 and demonstrates the value of implementing molecular markers to characterise materials in breeding pools of oat. Overall, our findings highlight the necessity of examining seed stocks using pedigree and molecular markers to ensure seed uniformity and bring robustness to surveillance methodologies.

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

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