First Report of Powdery Mildew Caused by Oidium neolycopersici on Tomato in China

Abstract

Tomato powdery mildew can cause remarkable reduction in fruit size and quality (4). In March of 2008, powdery mildew appeared as circular, white colonies on leaves, petioles, and stems of tomato plants grown in greenhouses in Shangqiu, Henan Province, China. The pathogenic fungus had unbranched conidiophores with an average length of 58.4 μm and width of 5.1 μm. Conidia were hyaline, elliptical, and were borne singly. Average length and width of conidia were 30.6 and 15.1 μm, respectively. Germ tubes were straight and formed at the ends or very close to the ends of conidia. Chasmothecium was not found in the collected samples. Different tomato cultivars and species, including Lycopersicon esculentum Mill (cvs. Moneymaker, Micro-Tom, Zaofen, Fenguo, and Zhongza series), L. peruvianum cv. LA2172, and L. hirsutum cv. G1.1560, were inoculated with a conidial suspension with a concentration of 5 × 104 conidia/ml. Plants developed powdery mildew symptoms as early as 4 days after inoculation. Susceptible symptoms developed on all L. esculentum cultivars, while L. peruvianum LA2172 and L. hirsutum G1.1560 displayed complete resistance, which is similar to the results of Bai et al 2004 (1) and Lindhout and Pet 1990 (3). Morphological characteristics of the pathogen on susceptible genotypes were similar to those from naturally infected plants. On the basis of the characteristics of the asexual stage, the pathogen was identified as an isolate of Oidium neolycopersici L. Kiss, which was confirmed by internal transcribed spacer (ITS) sequence analysis. PCR amplification and sequencing of the ITS region were performed with primers ITS1 and ITS4. The nucleotide sequence was assigned GenBank Accession No. EU486992, which had a 100% homology with 10 ITS sequences of O. neolycopersici in GenBank (Accession Nos. EU047559 to 047568) (2). In Asia, the spread of this pathogen has been recently reported in Japan (2). To our knowledge, this is the first report of tomato powdery mildew in China. Voucher specimens are available at the Specimen Center in the Department of Life Science, Shangqiu Normal University. References: (1) Y. Bai et al. Mol. Plant-Microbe. Interact. 18:354, 2005. (2) T. Jankovics et al. Phytopathology 98:529, 2008. (3) P. Lindhout and G. Pet. Tomato Gen. Coop. Rep. 40:19, 1990. (4) J. M. Whipps et al. Plant Pathol. 47:36, 1998.