The Phosphatase VdPtc3 Regulates Virulence in <i>Verticillium dahliae</i> by Interacting with VdAtg1-Reference-Cited by-同舟云学术

The Phosphatase VdPtc3 Regulates Virulence in Verticillium dahliae by Interacting with VdAtg1

Published:2022-11-30 Issue: Volume: Page:
ISSN:0031-949X
Container-title:Phytopathology®
language:en
Short-container-title:Phytopathology®

Author:

Liu Tao¹,Qin Jun¹,Shang Wenjing¹,Chen Jieyin²,Subbarao Krishna V.³,Hu Xiaoping⁴⁵

Affiliation:

1. Yangling, China;

2. Chinese Academy of Agricultural Sciences, Institute of Plant Protection, No. 2 Yuanmingyuan West Rd., Haidian, Beijing, [Select a State/Province], China, 100193;

3. University of California, Davis, Plant Pathology, 1636 E. Alisal St., Salinas, California, United States, 93905, , ;

4. Northwest A&F University, State Key Laboratory of Crop Stress Biology for Arid Areas, Xinong Road #22, Yangling, Shaanxi, China, 712100

5. Northwest A&F University, College of Plant Protection, Xinong Road #22, Yangling, Shaanxi, China, 712100;

Abstract

Type 2C protein phosphatases (PP2Cs) regulate various biological processes in eukaryotes. However, their functions in Verticillium dahliae have not been characterized. In this study, homologs VdPtc1, VdPtc3, VdPtc5, VdPtc6 and VdPtc7 were identified in V. dahliae based on homologous comparison with those in Saccharomyces cerevisiae. VdPtc2 and VdPtc4 are missing in the genome of V. dahliae XJ592 strain. VdPtc3 is the homologs of Ptc2, Ptc3 and Ptc4 proteins in S. cerevisiae, implying that VdPtc3 may play versatile functions in V. dahliae. VdPtc3 promoted conidium development, melanin and microsclerotium formation in V. dahliae. The ΔVdPtc3 strains showed increased sensitivity to NaCl and sorbitol and augmented the phosphorylation of p38 MAPK Hog1 induced by osmotic stress. Besides, the ΔVdPtc3 strains also showed milder Verticillium wilt symptom on cotton. Furthermore, uniquely to filamentous fungi, VdPtc3 interacts with VdAtg1, which modulates melanin and microsclerotium formation as well as pathogenicity.

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

Link

https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-09-22-0320-R