Abscisic Acid Is Involved in the Resistance Response of Arabidopsis thaliana Against Meloidogyne paranaensis

Abstract

Abscisic acid (ABA) is a classical hormone involved in the plant defense against abiotic stresses, especially drought. However, its role in the defense response against biotic stresses is controversial: it can induce resistance to some pathogens but can also increase the susceptibility to other pathogens. Information regarding the effect of ABA on the relationship between plants and sedentary phytonematodes, such as Meloidogyne paranaensis, is scarce. In this study, we found that ABA changed the susceptibility level of Arabidopsis thaliana against M. paranaensis. The population of M. paranaensis was reduced by 58.3% with the exogenous application of ABA 24 h before the nematode inoculation, which demonstrated that ABA plays an important role in the preinfectional defense of A. thaliana against M. paranaensis. The increase in the nematode population density in the ABA biosynthesis mutant, aba2-1, corroborated the results observed with the exogenous application of ABA. The phytohormone did not show nematicide or nematostatic effects on M. paranaensis juveniles in in vitro tests, indicating that the response is linked to intrinsic plant factors, which was corroborated by the decrease in the number of nematodes in the abi4-1 mutant. This reduction indicates that the gene expression regulation by transcript factors is possibly related to regulatory cascades mediated by ABA in the response of A. thaliana against M. paranaensis.