Effect of a Soil Amendment on the Survival of Ralstonia solanacearum in Different Soils

Abstract

The effect of a soil amendment (SA) composed of urea (200 kg of N per ha) and CaO (5,000 kg/ha) on the survival of Ralstonia solanacearum in four Philippine soils was investigated in a series of laboratory experiments. Within 3 weeks after application, the SA either caused an initial decrease, a final decline, or no change in the pathogen population, depending on the particular soil type. An initial decrease occurred in a soil with a basic pH and resulted in a significantly (P < 0.001) lower pathogen population immediately and at 1 week after amending the soil. This decrease was probably due to the high pH in the soil during urea hydrolysis. A final decline in the R. solanacearum population after 3 weeks occurred in two soils in which nitrite accumulated after 1 week. In these soils, no decline in bacterial levels occurred when nitrite formation was inhibited by 2-chloro-6-trichloromethylpyridine. In the soil with low pH, no nitrite accumulated and the R. solanacearum population did not decline. The suppressive effects of pH and nitrite on R. solanacearum growth were confirmed by in vitro experiments. Ammonium reduced the growth of R. solanacearum, but was not suppressive. Interactions of pH with ammonium and nitrite also occurred, whereby ammonium reduced growth of R. solanacearum only at pH 9 and nitrite was suppressive only at pH 5. Nitrate had no effect on R. solanacearum growth in vitro.