Author:
Sandal Niels,Petersen Thomas Rørby,Murray Jeremy,Umehara Yosuke,Karas Bogumil,Yano Koji,Kumagai Hirotaka,Yoshikawa Makoto,Saito Katsuharu,Hayashi Masaki,Murakami Yasuhiro,Wang Xinwang,Hakoyama Tsuneo,Imaizumi-Anraku Haruko,Sato Shusei,Kato Tomohiko,Chen Wenli,Hossain Md. Shakhawat,Shibata Satoshi,Wang Trevor L.,Yokota Keisuke,Larsen Knud,Kanamori Norihito,Madsen Esben,Radutoiu Simona,Madsen Lene H.,Radu Talida Gratiela,Krusell Lene,Ooki Yasuhiro,Banba Mari,Betti Marco,Rispail Nicolas,Skøt Leif,Tuck Elaine,Perry Jillian,Yoshida Satoko,Vickers Kate,Pike Jodie,Mulder Lonneke,Charpentier Myriam,Müller Judith,Ohtomo Ryo,Kojima Tomoko,Ando Shotaro,Marquez Antonio J.,Gresshoff Peter M.,Harada Kyuya,Webb Judith,Hata Shingo,Suganuma Norio,Kouchi Hiroshi,Kawasaki Shinji,Tabata Satoshi,Hayashi Makoto,Parniske Martin,Szczyglowski Krzysztof,Kawaguchi Masayoshi,Stougaard Jens
Abstract
Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.
Subject
Agronomy and Crop Science,General Medicine,Physiology