Virulence of novel Ralstonia pseudosolanacearum (phylotype I) strains from rose, blueberry and mandevilla on seed potato

Abstract

Potato (Solanum tuberosum L.) ranks fourth among the most important staple food in the world. Ralstonia solanacearum (phylotype (phy) IIB, sequevar (seq) 1 and 2), also known as R3B2, the causal agent of brown rot disease on potato, is extremely damaging, causing great economical losses to potato in temperate regions. It is thought that members of Ralstonia pseudosolanacearum (phy I) are not pathogenic at low temperatures and usually are found in warmer climates. R. pseudosolanacearum strain PD 7123 (seq 33) isolated from roses in the Netherlands, strain P824 (seq 13) isolated from blueberry and strain P781 (seq 14) from mandevilla in Florida are phylogenetically closely related and could share the same host. The virulence and ability of these novel strains to multiply latently in potato in temperate regions is unknown. The objective of this work was to assess the virulence and presence of latent infections of the mentioned R. pseudosolanacearum strains on three commercial seed potato cultivars under warmer (28℃) and temperate (20℃) temperatures. At 28℃ all three R. pseudosolanacearum strains caused severe symptoms on all potato cultivars. Overall disease severity on potato was lower at 20℃ than 28℃, however, major differences in virulence of the three strains were observed at 42 dpi among potato cultivars. All asymptomatic potato plants and most of their daughter tubers had latent infections at 20℃. Altogether, these results show that the phy I strains from rose, blueberry and mandevilla may pose a threat to potato production in temperate climates and the worldwide movement of seed potatoes.