Efficacy of Fluazinam in Suppression of Monosporascus cannonballus, the Causal Agent of Sudden Wilt of Melons

Abstract

Sudden wilt (vine decline) of melon, caused by Monosporascus cannonballus, is a worldwide problem in arid and semi-arid regions. Soil disinfestation by fumigation with methyl bromide before planting is a common treatment for disease management but, because methyl bromide is expected to be banned from use within the next 10 years, alternative measures for disease suppression are needed. The efficacy of 29 fungicides against M. cannonballus was evaluated in vitro. Among the fungicides tested, fluazinam and kresoxim methyl were the most effective and both totally inhibited the growth of M. cannonballus in culture at concentrations of 10 μg a.i /ml. Because fluazinam also was effective in inhibition of Pythium aphnidermatum, which also may be involved in sudden wilt syndrome, and kresoxim methyl was not, fluazinam was chosen for further tests. The effective dose of fluazinam for M. cannonballus that reduced mycelial growth by 50% was 0.09 μg a.i./ml. Fluazinam efficacy was evaluated in three field experiments conducted in the spring and in the late summer cropping seasons. In two of the experiments, applications of fluazinam resulted in approximately 87% wilt reduction, whereas in the third experiment it was only 32%. The mobility of fluazinam in soil was determined in samples taken from the field. Fungicide mobility in soil was relatively limited; most of the compound was adsorbed to soil particles, resulting in a zone of high concentration that decreased with depth and distance from the application site. Nevertheless, rates measured even at a depth of 25 cm were sufficient to control M. cannonballus. This study shows that fluazinam may be used as one component in an integrated approach for suppression of sudden wilt of melons.