Reaction of Broccoli to Isolates of Verticillium dahliae from Various Hosts

Abstract

Isolates of Verticillium dahliae from 15 different hosts and V. albo-atrum from alfalfa were tested for their ability to cause wilt on broccoli using a root-dip inoculation method. None of the isolates caused vascular discoloration in broccoli except those from cabbage and cauliflower that were weakly pathogenic. Broccoli cultivars Baccus, Greenbelt, Parasol, Patriot, and Symphony showed resistance to Verticillium infection. Re-isolated strains from fresh samples of internally discolored broccoli and cauliflower root tissues were unable to cause disease symptoms on re-inoculation of broccoli, but caused severe disease on cauliflower. Inoculation of 5-, 7-, 9-, or 11-week-old plants did not alter the resistance in broccoli or the susceptibility in cauliflower against Verticillium. Immunity of broccoli against infection by isolates of V. dahliae from non-crucifer hosts, its resistance against crucifer isolates, and as previously described, attrition of V. dahliae microsclerotia in soil by broccoli residue, coupled with its importance as a commercial vegetable, make broccoli an attractive rotation crop for the management of Verticillium wilt in many cropping systems.