Transmission of Erwinia stewartii from Plants to Kernels and Reactions of Corn Hybrids to Stewart's Wilt

Abstract

Stewart's wilt reactions of 98 food-grade, white corn hybrids, 3 yellow dent corn hybrids, and 23 sweet corn hybrids and infection of kernels by E. stewartii were evaluated in 1998, 1999, and 2000. Stewart's wilt symptoms were rated from 1 (no appreciable spread of symptoms) to 9 (dead plants) following inoculation. The mean Stewart's wilt ratings for the food-grade, white corn and yellow dent corn hybrids were 1.9, 2.4, and 2.9 in 1998, 1999, and 2000, respectively. The mean Stewart's wilt ratings for the sweet corn hybrids were 3.8, 4.2, and 4.6 in 1998, 1999, and 2000, respectively. Hybrids with ratings less than 3 were classified as resistant. Hybrids with ratings between 3 and 4.5 were classified as moderate. Hybrids with ratings greater than 4.5 were classified as susceptible. Ears harvested from each row in 1998 and 1999 were assayed for E. stewartii using an enzyme-linked immunosorbent assay (ELISA)-based seed health test. Kernels from 16 hybrids were positive for E. stewartii in 1998. Kernels from 11 hybrids were positive for E. stewartii in 1999. Kernel infection by E. stewartii was affected considerably by the level of host resistance (i.e., reactions of seed parent plants). For hybrids classified as resistant, estimates of kernel infection were 0.024 and 0.0007% in 1998 and 1999, respectively. For hybrids with moderate reactions to Stewart's wilt, estimates of kernel infection were 0.19 and 0.07% in 1998 and 1999, respectively. For hybrids with susceptible reactions to Stewart's wilt, estimates of kernel infection were 11.6 and 7.8% in 1998 and 1999, respectively. Based on high levels of Stewart's wilt resistance in food-grade, white corn hybrids, and low rates of kernel infection by E. stewartii in resistant and moderate hybrids, there is an exceedingly low probability of introducing E. stewartii to areas where it does not occur by transmitting the bacterium in grain of the food-grade, white corn hybrids evaluated in this study. Although all of the kernels harvested in these experiments were produced as grain on open-pollinated F1 hybrids, the rates of kernel infection observed for hybrids with resistant, moderate and susceptible reactions to Stewart's wilt are applicable to seed produced on inbred lines with equivalent Stewart's wilt reactions.